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所在平台: Udemy |
课程主页: https://www.udemy.com/course/understanding-cell-cycle-cancer-and-monoclonal-antibodies/
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课程名称：肿瘤学与单克隆抗体 课程概述：本课程将深入探讨正常细胞周期的神奇机制，讲解其在正常条件下的运作方式。同时，将分析细胞周期调控机制失效所导致的癌症发展。此外，我们还将探索单克隆抗体这一前沿领域，以及它们如何成为癌症治疗的强大工具。本课程为学生和专业人士设计，内容涵盖以下几个重要方面： 1. **细胞周期**：了解细胞周期的不同阶段及其所需的机械装置（如中心粒、动粒和微管），确保细胞正确分裂。掌握细胞周期控制系统的重要角色，包括细胞周期蛋白、细胞周期依赖性激酶和受控降解机制（泛素-蛋白酶体系统）。 2. **p53的角色**：了解p53蛋白如何作为基因组的守护者，确保子细胞获赠完整的遗传物质副本，并在DNA受损时停止细胞周期。 3. **癌症基础**：学习肿瘤的基本概念、命名和分类，区分良性和恶性肿瘤的不同。 4. **致癌作用**：探讨致癌物和共同致癌物在癌症发展中的作用，理解原癌基因、癌基因和肿瘤抑制基因的差异及其工作机制。 5. **正常细胞与癌细胞**：探讨正常细胞与癌细胞之间的根本差异。学生将学习癌症分期系统（TNM系统和编号系统）。 6. **单克隆抗体与癌症治疗**：了解抗体的结构（重链和轻链、铰链区、互补确定区）以及B细胞受体和分泌性抗体的区别。学习什么是单克隆抗体及其特征，了解单克隆抗体的生产流程，包括杂交瘤技术的应用，如何使用HAT培养基筛选所需的杂交瘤，采用ELISA和亲和色谱等技术筛选杂交瘤的功能。探索各种用于癌症治疗的单克隆抗体类型，包括裸抗体、 conjugated抗体和双特异性抗体，以及具体使用的单克隆抗体如Alemtuzumab和Brentuximab vedotin。 本课程为希望深入了解肿瘤学和单克隆抗体的学习者提供了丰富的知识基础与实践应用。
Course Description: Oncology and Monoclonal AntibodiesCourse OverviewThis course will focus on the fascinating mechanisms of the normal cell cycle, explaining how it functions under typical conditions. You'll also learn what happens when the regulatory controls of the cell cycle break down, leading to the development of cancer. Additionally, we will explore the cutting-edge world of monoclonal antibodies and how they can be used as a powerful treatment for cancer. This comprehensive course designed for students and professionals alike with the following details of the contents of the course.The Cell CycleExplore the different phases of the cell cycle and the machinery (centrosomes, kinetochores and microtubules) that ensures proper cell division.Understand the pivotal roles of the control system of the cell cycle that includes cyclins, cyclin-dependent kinases and the regulated destruction machinery (Ubiquitin Proteasome System)The Role of p53Discover how the p53 protein serves as a guardian of the genome, and how it make sure that the daughters cells will receive an intact copy of the genetic material by stopping the cell cycle when there is damage to the DNA.Cancer BasicsBasics of the Neoplasm/tumor and the nomenclature and classification of the tumor.What are the differences between benign and malignant tumors.CarcinogenesisWhat are the roles of carcinogens and co-carcinogens in cancer development.Understand the differences in protooncogenes, oncogenes, and tumor suppressor genes and how they work.Normal vs. Cancer CellsUnderstand the fundamental differences between normal and cancer cells.The students will explore the cancer staging system (The TNM system and the numbering system)Monoclonal Antibodies and Cancer TreatmentThe Structure of the Antibody; Heavy and Light Chains, Hinge Region, CDRThe B-cell Receptor and the Secretory Antibodies and their Differences What are monoclonal antibodies and their characteristics How the monoclonal antibodies are produced by the hybridoma technology How to select the desired hybridoma, from the mixture of hybridoma, using HAT media.How to screen the hybridoma for their desired functions using different techniques like ELISA and affinity chromatographyDiscover the various types of monoclonal antibodies used for the treatment of cancer; naked, conjugated and bispecific and the use of different monoclonal antibodies like Alemtuzumab, Brentuximab vedotin
