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所在平台: Udemy |
课程主页: https://www.udemy.com/course/ucancrispr-online-master-class/
课程评论：没有评论
课程名称：UcanCRISPR在线：CRISPR/Cas9大师班 课程概述：本课程提供一个大学水平的CRISPR/Cas9深入研究，且价格合理。课程内容覆盖CRISPR的广泛主题及最新发展，包括多个预览讲座，以帮助学生判断该课程是否适合自己。课程主要内容包括以下四个部分： 1. **CRISPR及其功能基础介绍**：重点学习最常用的CRISPR系统Cas9的基本组成和功能，了解其作为适应性免疫系统如何保护细菌和古菌免受病原DNA的侵害，以及从不同基因中演变而来的多种变体。 2. **技术应用探索**：探讨CRISPR技术的应用，包括通过靶向核酸酶实现基因功能破坏，以及使用改造后的非切割系统进行其他类型的基因修改。还将介绍识别CRISPR造成的基因改变的主要筛选程序。 3. **快速发展的影响**：讨论CRISPR技术的快速进展及其深刻理解，内容包括使用蛋白质结构模型对Cas9蛋白进行工程改造。探讨CRISPR如何存储基因组中的新序列，以及如何以新方式响应细胞状态的变化，并考虑各种应用案例的后果。 4. **CRISPR系统设计**：讨论为不同应用案例设计CRISPR系统的过程，包括在体表达和纯化复合物的使用。通过两个示范，展示识别感兴趣基因的靶位点及制作定制化sgRNA的方法。 学习目标： - 理解CRISPR作为一种自然现象的基本概念。 - 学习基于CRISPR平台的不同技术。 - 评估在各种宿主生物体中进行不同CRISPR实验的方法。 - 运用所学知识设计定制的引导RNA，并确定如何获得相关材料。 欢迎访问我们的平台页面，以获取免费直播课程的信息、课程折扣码等更多内容。
Visit our platform page in my bio to get information on free live classes, a discount code for this course, and more.This is a college level course for a fraction of the cost. There is no other course that covers the breadth of CRISPR and the latest developments. There are several lectures for preview that will give you a good idea if this course is for you.This course will be an in-depth study of CRISPR/Cas9 as a natural phenomenon, applications, and implications, this course is unique in covering the latest understanding and engineering of CRISPR reported in scientific literature. Four sections to cover the major areas of CRISPR include:1. Introduction of the basic components and function of the most widely utilized CRISPR system, Cas9. The CRISPR adaptive immune system protects bacteria and archaea from pathogenic DNA, and has evolved from from several interesting genes into a number of variants. 2. An exploration of the applications of this technology. You will learn about gene disruption by targeted nucleases, and applications of modified non-cutting systems for making other types of modifications. The section starts by introducing the major types of screening procedures used to identify changes made by CRISPR.3. Implications of the rapid developments and deeper understanding of CRISPR currently taking place. Starting with using protein structure models to engineer the Cas9 protein. CRISPR can store new sequence in a genome and respond to alterations in cell state in novel ways. Since this technology is becoming very widely available the ramifications of different use cases will be considered. 4. A final section will discuss designing CRISPR systems for different use cases, including in vivo expression and use of purified complexes. Two demonstrations of identifying target sites in genes of interest, with methods to produce the customized sgRNA are made.Learning objectives:Develop understanding of CRISPR as a natural phenomenon.Learn the different technologies built on the CRISPR platform.Be able to assess approaches to different CRISPR experiments in a variety of host organisms.Use the knowledge to design custom guide RNAs and determine how to obtain the materials.
