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所在平台: Udemy |
课程主页: https://www.udemy.com/course/the-introduction-to-mammalian-cell-culture-biotechnology/
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**课程名称：哺乳动物细胞培养生物技术导论** **课程概述：** 本课程专为有志于学习细胞培养但不知从何入手的天才生物技术专业本科生和研究生设计。本课程将成为您开启研究项目的有力助手，特别适合拥有生物学背景，尤其是生物技术、分子生物学、细胞生物学、微生物学、蛋白质组学及相关领域知识的学生。课程无任何先修要求，您将从最基础的层面全面掌握知识。本课程面向对生物技术领域的哺乳动物细胞培养概念感到陌生，几乎没有或完全没有相关知识或经验的初学者。 **课程目标：** 本课程旨在提供哺乳动物细胞培养基础知识的全面概述。 **课程内容：** * **第一课：细胞培养基础。** 介绍细胞培养的定义、培养方式、原代培养与传代培养的区别，以及细胞培养在生物技术领域的重要性与应用。 * **第二课：细胞株介绍。** 学习不同类型的细胞株及其相关示例。 * **第三课：细胞培养实验室准备。** 讲解细胞培养实验所需的基本条件和关键实验耗材。 * **第四课：实验室安全、无菌技术与污染防控。** 探讨实验室安全挑战、无菌操作技术以及各种常见的细胞培养污染问题。 * **第五课：细胞复苏、冻存与传代。** 学习基础的细胞融解、低温保存和传代培养操作流程。 **重要提示：** 尽管细胞培养实验的基础知识存在共性，但不同的细胞类型和实验室在细胞培养的条件上存在显著差异。改变特定细胞系的培养条件可能导致其表现出不同的表型。因此，研究人员务必熟悉自己感兴趣的细胞系，并严格遵守实验操作说明及所用产品的指导。 **相关标签：** #生物技术 #微生物学 #分子生物学 #免疫学 #组织培养 #细胞培养 #生物技术 #遗传学 #生物信息学 #生物学 #生物化学 #基因编辑 #纳米技术 #临床研究
This course is designed for undergraduate and graduate biotechnology students who possess an inclination towards acquiring knowledge in cell culture but lack a starting point. This resource will primarily assist students with a background in biology, particularly in the fields of biotechnology, molecular biology, cell biology, microbiology, proteomics, and related disciplines, in initiating their research projects. This course does not have any prerequisites. You will acquire comprehensive knowledge starting from the fundamental level. This course is designed for individuals who are new to the concept of cell culture in the field of biotechnology and have little to no prior knowledge or experience.The purpose of this course is to provide an overview of the fundamentals of mammalian cell culture. The initial lesson of the program provides a fundamental introduction to cell culture, including definitions, varieties of cultures, distinctions between primary and secondary cultures, as well as the significance and applications of cell culture in the field of biotechnology. The second lesson introduces the different types of cell lines, along with their examples. The third lecture covers the prerequisites and essential supplies for a laboratory devoted to cell culture experiments. The fourth lecture covers challenges in laboratory safety, aseptic technique, and various cell culture contaminations, while the final lecture covers fundamental cell thawing, cryopreservation, and subculturing procedures. Although there are certain similarities in the fundamentals of cell culture experiments, the conditions under which cells are cultured differ significantly between labs and cell types. Altering the culture conditions specified for a given cell line may lead to the expression of distinct phenotypes. Consequently, it is advisable that researchers acquaint themselves with the cell line of interest and diligently adhere to the instructions included with every product utilized in their experiments.#biotechnology #microbiology #molecular biology #immunology #tissue culture #cell culture #biotech #genetics #bioinformatics #biology #biochemistry #gene editing #nanotechnology #clinical research
