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所在平台: Udemy |
课程主页: https://www.udemy.com/course/the-basic-principles-of-gene-cloning-and-dna-analysis/
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课程名称：基因克隆与DNA分析的基本原理 课程概述：基因克隆是生物技术和现代分子生物学中最重要的技术之一。这种技术曾被用于人类基因组计划，成功确定了几乎所有人类染色体的遗传内容序列。此外，第一个商业化的重组产品“胰岛素”也是基因克隆的成果。基因克隆在各种研究中的应用广泛，且在工业上具有重要意义。因此，了解基因克隆的基本概念及其工作原理显得尤为重要。该课程以T.A. Brown的《基因克隆与DNA分析》为基础，以简单易懂的方式描述基因克隆的基本原理。课程包括多个讲座，内容涵盖用于基因克隆的载体、DNA的纯化、重组DNA分子的合成、DNA导入活细胞的方法、转化细胞的选择、所需重组体的选择、聚合酶链反应以及利用凝胶电泳进行的DNA分析。每个模块至少包含一次测验，帮助学员检测在各自模块中获得的知识。此外，课程还包括期中考试和期末考试，每个考试都有实践测试和作业。实践测试尤其重要，因为与测验不同，实践测试强调知识的应用。
Gene cloning is one of the most important techniques in biotechnology and modern molecular biology. It is the same technique that was used in the Human Genome Project that successfully determined the sequences of almost all the genetic content of the chromosomes of humans. Moreover, the very first commercially available recombinant product "Insulin" was also the result of gene cloning. The technique has been used in a wide variety of studies and has great importance from the industrial point of view as well. It is, thus, important to have an idea of what gene cloning is and how it works. This course describes the basic principles of gene cloning in a simple way and is based on the book "Gene Cloning and DNA analysis" by T.A. Brown. The course has a number of lectures on topics such as vectors used for gene cloning, purification of DNA, synthesis of recombinant DNA molecules, the introduction of DNA into living cells, selection of the transformed cells, selection of the desired recombinants, polymerase chain reaction and DNA analysis using gel electrophoresis. Each section/module has at least 1 Quiz in order to test the knowledge that you'll gain in the respective section/module. Apart from lectures and quizzes, the course consists of a mid-term and a final term; each of which consists of a practice test and an assignment. Practice tests are of particular importance as, unlike quizzes, they are not based on the knowledge but on the application of the knowledge that you'll gain in this course.
