|
所在平台: Udemy |
课程主页: https://www.udemy.com/course/practical-bioinformatics-i/
课程评论：没有评论
课程名称：实用生物信息学 I 课程概述：本课程旨在通过学习多种工具和操作方法，帮助学员准确理解生物信息学文件及其组成和输出，从而拓展对生物信息学应用的认识。课程主要围绕核酸的实际应用展开，使用的程序大多是流行的、免费的在线工具，或适用于所有操作系统的软件。 课程内容分为以下几部分： 第一部分：DNA桑格测序 - 介绍桑格测序方法及其重要性。 - 讨论桑格测序生成的文件、常见问题及成因。 - 实际操作，包括使用三种不同程序打开和处理桑格测序文件。 第二部分：高通量测序 - 讨论常见的高通量测序方法，如Illumina、Ion Torrent、太平洋生物科学公司和牛津纳米孔。 - 探索高通量测序在不同领域的各种应用。 第三部分：生物信息学文件 - 介绍生物学者使用的原始数据文件，包括序列、比对、变异和注释等，主要文件类型包括：FASTQ、FASTA、Genbank、GTF/GFF3、BED、SAM/BAM、BCF/VCF。 第四部分至最后一部分：实用应用 - 使用在线软件包对上述各种文件进行实际操作，例如： - 序列处理套件 2（Sequence Manipulation Suite 2） - EMBOSS - Galaxy 平台上的包：Seqtk、Bedtools、Samtools、Bcftools。 课程中包含许多程序，其中一些的基本操作已做详细讲解，而其他程序则帮助学员更好地理解示例，虽然未详细讲解，但用于特定功能的执行。题库尚未完成。
Learning many tools and manipulation methods helps you to accurately understand files, their components, and their outputs, thus broadening your horizons in bioinformatics applications in general.Practical applications will be on nucleic acidsThe programs used are popular, free, and online (majority) or software installed on all operating systemsThe first part discusses the DNA Sanger Sequencing because of its great importance so far despite the old method, and we will talk in this part about:Explanation of the Sanger Sequencing method, what files it produces, problems, and their causes.A practical application to open and manipulate the Sanger Sequencing files using three different programs.The second part discusses High-throughput Sequencing, which is the basis of recent nucleic acid analysis research, and we will talk in this part about:Common High-throughput Sequencing methods are Illumina, Ion Torrent, Pacific Biosciences, and Oxford Nanopore.Various applications of High-throughput Sequencing in different fields.The third part discusses bioinformatics files, which are the raw material for biologists where sequences, alignments, variations, and annotations are stored. The files mainly found are:FASTQFASTAGenbankGTF/GFF3BEDSAM/BAMBCF/VCFParts from the fourth to the last are practical applications on the aforementioned files using various programs from online packages such as:Sequence Manipulation Suite 2.EMBOSS.Packages on the Galaxy platform:Seqtk.Bedtools.Samtools.Bcftools.The course contains many programs, some of which are basic in manipulation and have been explained in detail, and other programs were found to help clearly understand the examples, and these were not explained in detail but were used to perform a specific function.The quizzes are not yet complete.
