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所在平台: Udemy |
课程主页: https://www.udemy.com/course/pcr-to-multiplex-pcr-and-sequencing/
课程评论：没有评论
**课程名称:** 构筑坚实基础——PCR、多重PCR和DNA测序 **课程概述:** 本课程旨在帮助您巩固PCR知识，学习新的PCR技巧，提升PCR技能，并将其应用于荧光DNA测序。课程涵盖了从引物设计到PCR凝胶分析的整个流程，并会分享在实际操作中积累的经验和改进方法。您将学习如何处理宝贵的样本（直至毛细管电泳步骤），以及如何解读Sanger测序获得的基因序列图谱。课程还将详细介绍PCR失败的排查和解决步骤。 **目标学员:** * 生物学、生物技术、分子生物学（本科及硕士）学生 * PCR实验室、科研实验室的本科实习生 * 遗传学/分子学/医学实验室专业人员 **课程特色:** * **一站式学习:** 从引物到PCR凝胶分析，全面覆盖。 * **实践导向:** 提供真实实验图像、案例和在线演示，易于理解和操作。 * **数据资源:** 学习如何利用NCBI等在线数据库检索基因信息，并将序列处理成FASTA格式，用于引物设计和序列分析（例如使用NCBI primer-BLAST工具）。 * **解决问题:** 教授解决PCR失败问题的详细方法。 * **互动学习:** 提供学习复习题、作业和练习测试，并保证讲师的个人关注，解答学员疑问。 **讲师寄语:** PCR是分子生物学的基础，也是许多其他分子技术发展的基石。我很乐意把我多年的实践经验和“试错”中学到的技巧分享给大家，帮助大家在PCR领域取得成功。期待您的加入！
Do you intent to refresh your PCR knowledge and also learn new, unaware PCR tips? Small steps you take could lead to success in PCRAre you planning to upgrade your PCR skills to higher level with new possibilities? increase per PCR outputDo you want to take advantage of PCR skills for fluorescent DNA sequencing application? not handing over your precious samples completely(Master sample processing upto capillary electrophoresis step, if electrophoresis is done elsewhere outside your lab)How to visualize your gene sequence graphs after Sanger sequencing? available free to download sequence visualization toolsCurious how PCR failures are handled to resolve the issue? Step-by-Step detailed methodology givenLook no further and enrol now and see for yourself.The course is for Biology, Biotechnology, Molecular biology (undergraduate and Master's) studentsUndergraduate interns at PCR laboratories, research labsGenetic/Molecular/Medical laboratory professionals What motivated me to construct the coursePCR is the foundation of molecular biology which led to development of other molecular methods.Felt disconnect between PCR practical knowledge and traditional classroom instruction.The tips and PCR improvements learned through my trial and error methods during my PCR journeyThe learning format include Complete one-stop shop- Primers to PCR gel analysis Study review questions, assignments and practice testsImages from real-bench work, examples and web-based demonstrations for easy following. Example of using well established molecular genetic resource to retrieve gene informationLearn the ten steps for confident PCR Acquire skills in researching and gathering genomic data from web-based Databases(NCBI as example), processing the gene sequence to FASTA format for primer design or sequence analysis( NCBI primer-BLAST tool)I guarantee my personal attention to your queries during your learning experience and enthusiastic about ensuring a good learning experience for you now and in future. You could also add the course to your wish list for future enrollment.Thank you and see you soon after enrollment. Biju Joseph
