|
所在平台: Udemy |
课程主页: https://www.udemy.com/course/molecular-biology-and-biotechnology-principles/
课程评论：没有评论
**分子生物学与生物技术原理课程总结** 本课程深入探讨了分子生物学和生物技术的核心原理，涵盖了以下关键领域： **第一单元：重组DNA技术** 本单元重点介绍重组DNA技术，包括： * **关键工具：** 详细阐述了用于重组DNA技术的主要工具。 * **基因与载体分离：** 讲解了如何分离载体DNA和目标基因。 * **基因转移方法：** 探讨了将外源基因导入细菌细胞的各种方法。 * **限制性内切酶与连接酶：** 介绍了这两种在DNA重组中至关重要的酶的作用。 * **载体类型：** 讨论了Cosmid、质粒等载体的特性。 * **应用实例：** 以胰岛素基因的生产为例，展示了重组DNA技术的实际应用。 **第二单元：分子生物学技术** 本单元重点介绍两种重要的分子生物学技术： * **Southern Blotting：** 用于DNA样品的分析。 * **Northern Blotting：** 用于RNA样品的分析。 **第三单元：杂交技术与电泳** 本单元涵盖了以下技术： * **杂交技术：** 介绍了DNA/RNA分子之间的杂交原理。 * **聚合酶链式反应 (PCR)：** 讲解了PCR如何高效扩增DNA样本。 * **放射免疫分析 (RIA)：** 介绍了基于抗原-抗体特异性结合的检测方法。 * **电泳技术：** * **基本原理：** 阐述了电泳基于分子大小和所带电荷进行分离的原理。 * **具体应用：** 介绍了Southern blotting、Northern blotting、凝胶电泳（用于DNA/RNA分离）、SDS-PAGE（用于蛋白质和氨基酸分离）、纸电泳等多种电泳技术。 **第四单元：色谱技术** 本单元深入介绍各种色谱技术： * **基本原理：** 阐述了色谱技术通过分子大小、电荷和分子量等特性进行分离的原理。 * **特定技术：** * **柱层析：** 介绍了基于样品与固定相相互作用的分离。 * **亲和层析：** 讲解了基于配体与蛋白质特异性亲和力进行分离的原理。 * **离子交换层析：** 区分了阳离子交换和阴离子交换，以及基于蛋白质样品中离子性质进行分离的原理。 * **气液色谱：** 介绍了以气体为流动相、液体为固定相的分离方法。 * **纸层析：** 介绍了以纸为固定相，利用试剂识别样品中化合物的原理。 * **分离机制：** 总结了基于抗原-抗体相互作用、酶与配体相互作用等特异性相互作用进行大分子分离的原理。 **课程特色：** 本课程将根据学生在问答环节提出的问题，不断进行内容补充和完善，以提供更全面的学习体验。
In this course students will learn about molecular biology and biotechnology principles.1. Unit 1Topics covered in: Lecture 1 , 2 and 3Recombinant DNA technology Tools used in Recombinant DNA technologyIsolation of vector gene Isolation of desired geneMethods to transfer foreign gene into bacterial cellDiscuss about Restriction enzymesAnd about ligases Vectors like cosmid, plasmid Production of insulin gene Applications of recombinant DNA technology2. Unit 2Topics covered in Lecture 4 Molecular biology TechniquesSouthern blotting technique used for DNA samplesNorthern blotting used for RNA samples 3. Unit 3Topics covered in Lecture 5Hybridisation technique Polymerase chain reaction used to amplify the DNA in the sample Radio immuno Assay - Antigen and antibody specific Electrophoresis zone electrophoresis Gel electrophoresis Polyacrylamide gel electrophoresis.Paper electrophoresis.Principle of electrophoresis.Used to separate the DNA depending on the size and charge on the molecule.Gel electrophoresis - is used to separate DNA or RNA.SDS page used to separate proteins and amino acids4. Unit 4Topics covered in Lecture 6 and 7 chromatographycolumn chromatography Affinity chromatography.- based on affinity towards ligand and protein.Ion exchange chromatography.Cat-ionic exchanger and anionic exchangerSeparation is based on ions present in the protein sample Gas liquid chromatography. Gas acts as a mobile phase and liquid acts as a stationary phase.gases like inert gases are used and liquid of high boiling point is used in gas liquid chromatographyPaper chromatography. Paper is used and reagent are used to identify the compound present in the sample principle of chromatography separation of molecules based on size, charge and molecular weight Separation of macro molecules based on antigen and antibody interactionEnzyme and ligand interaction as there is a specific interaction between the antigen and antibody interaction Summary of the courseAdd-onCourse would be added/enhanced based on Q & A form students in to form of additional information
