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所在平台: Udemy |
课程主页: https://www.udemy.com/course/molbio-expert-y-learn/
课程评论：没有评论
**Coursera - 分子生物学技术课程总结** 本课程专注于分子生物学的核心技术，旨在为学生提供坚实的理论基础和实践知识。 **课程核心内容：** * **DNA提取与纯化：** 课程详细介绍了植物DNA的纯化方法，重点讲解了有机溶剂（如氯仿、苯酚、苯酚:氯仿:异戊醇混合物）在去除蛋白质、实现核酸沉淀中的作用。纯化后的DNA应适用于限制性酶切、PCR扩增和克隆等下游实验。 * **核酸基础知识：** 解释了核酸（DNA和RNA）的本质——由核苷酸构成的生物大分子，并介绍了核苷酸的组成（糖、磷酸基团、含氮碱基）。强调了理解核酸这一基础的重要性。 * **基因组概念：** 引入了“基因组”的概念，即真核生物细胞核内完整的DNA分子集合。 * **DNA结构：** 阐述了DNA的双螺旋结构，特别是两条链之间通过碱基配对的氢键连接。 * **琼脂糖的性质：** 介绍了琼脂糖是一种从海藻中提取的线性聚合物，在室温下不溶于水或缓冲液，但在煮沸后可溶解，为电泳奠定基础。 * **电泳技术：** 讲解了电泳作为一种分离带电分子的关键技术。 * **课程目标：** 整体目标是让学生全面掌握分子生物学的基本知识。 **教学方式与特色：** * **视频内容：** 超过90%的内容以10分钟以内的短视频形式呈现，内容精炼，概念清晰。 * **视觉辅助：** 配备图片，直观地展示和解释相关概念。 * **教学手段：** 采用PPT、预录讲座和测验相结合的方式进行教学。 * **应用导向：** 课程内容将结合实际的生物学应用进行讲解。 * **备考支持：** 课程完成后，学生将能够充分准备相关考试。 **本课程适合对象：** 希望深入了解分子生物学基本原理和技术，并为相关学术或职业发展做准备的学生。
The isolation of pure DNA is a first step in the progress of molecular studies in plant. Organic solvents such as chloroform, phenol or a mixture of phenol: chloroform: isoamyl alcohol (25:24:1) are used for denaturation and precipitation of proteins from nucleic acid solution and denatured proteins removed by centrifugation. The isolated DNA should be suitable for restriction digestion, amplification, cloning.To understand the basic techniques use to work with nucleic acids, remember that nucleic acids are macromolecules made up of nucleotides ( a sugar, phosphate group, nitrogenous base ).An entire set of DNA molecule in the nucleus of eukaryotic oraganism is called genome.DNA has complementary strands linked by hydrogen bonds between the paired bases.Agarose is a linear polymer extracted from seaweed.Purified agarose is a powder insoluble in water or buffer at room temperature but dissolves on boiling.Electrophoresis is a technique used to seperate charged molecules.The overall goal of course is to give student knowledge of molecular biology.More than 90% of content presented in short videos not longer than 10 minutes that concisely explains concepts.Pictures are attached to explore the concepts briefly.This course will be taught through ppt, pre- recorded lectures and quizzes.This course will taught with applications of real world.After completing this course you would be able to fully prepare for examination.
