|
所在平台: Udemy |
课程主页: https://www.udemy.com/course/mass-spectrometry-ms-technique-the-must-have-basics/
课程评论：没有评论
课程名称：质谱 (MS) 技术：必须掌握的基础知识 课程概述：本课程重点区分光谱学与质谱学。光谱学是理论科学，而质谱学则是对原子和分子层面物质平衡的实际测量。光谱学研究物质与辐射能量之间的相互作用，主要关注物质在电磁辐射下的吸收行为，属于理论探索；而质谱学则是获取光谱定量测量的方法。质谱（MS）作为质谱学的一种，是一种分析技术，可以在高真空下测量化学样品中自由离子的质量，从而确定分子的元素组成和化学结构。 质谱的过程通常包括通过电子束对粒子进行电离，再将其置于磁场中以分离不同质荷比的粒子。分离后的粒子通过电子倍增器进行测量，从而根据每个离子的质量识别样品的组成。为了理解质谱，需要掌握离子的生成过程，因此课程展示了质谱技术的关键部分，主要面向具备基本分析化学知识的初学者。 课程从简洁的介绍开始，阐述该技术的原理，尤其是最重要的“裂解反应”，以及如何生成离子。课程提供逐步讲解，培养学员进行任何分子的裂解及解析质谱的能力。所有课程内容都通过例子和练习题进行强化，帮助学员实践质谱的解读。
Spectrometry VS Spectroscopy!Spectroscopy is thetheoretical science, and spectrometry is the practical measurement in the balancing of matter in atomic and molecular levels.Spectroscopy is the science of studying the interaction between matter and radiated energy. It's the study of the absorption behaviour of matter, when subjected to electromagnetic radiation. It doesn't generate any results, it's simply the theoretical approach to science. On the other hand, spectrometry is the method used to acquire a quantitative measurement of the spectrum. Mass spectrometry (MS) is an example of a type of spectrometry. It is an analytical technique that measures masses of free ions in high vaccum within a chemical sample and for detrmining the elemental composition and chemical structures of molecules. This is usually done by ionising particles with a shower of electrons, then passing them through a magnetic field to separate them into different stages of deflection. Once the particles are separated, they're measured by an electron multiplier, and we can identify the makeup of the sample through the weight of each ion's mass. In order to understand mass spectra, knowledge is necessary on how the ions were created. Therefore, the course exhibits the key parts of MS technique. It is basically intended for beginners with basic knowledges in analytical chemistry. The course starts with a concise introduction, represents the principles of this technique and most importantely how the ions are created through the most important "fragmentation" called reactions.A step-by-step explanation is given in order to develop the ability to perform fragmentation of any molecule and analyze any MS spectra. All sessions are reinforced with examples and problems in order to practice the interpretation of MS spectra.
