Learn HPLC samples preparations and calculations

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课程主页: https://www.udemy.com/course/learn-hplc-samples-preparations-and-calculations/

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课程简介

**课程名称:** 高效液相色谱 (HPLC) 样品制备与计算 **课程简介:** 本课程是一门面向药物质量控制领域的进阶课程,专注于高效液相色谱 (HPLC) 的样品制备和计算。课程强烈推荐给具有至少六年质量控制实验室经验的分析师。 **课程内容概述:** * **流动相制备:** 详细讲解缓冲溶液制备、pH 调节、等度和梯度洗脱模式的加载,以及标准溶液制备序列的创建。 * **测定样品制备:** 涵盖测定用溶液和标准溶液的制备。 * **测定结果计算:** 学习使用合适的方程计算测定结果,并提供实例。 * **有关物质 (Related Substances) 详解:** * 介绍有关物质的概念。 * 讲解所有有关物质测试所需溶液的制备方法。 * 深入学习有关物质的各类计算方法及其对应的方程,并辅以计算实例。 * 强调制备有关物质溶液时需要遵循的关键注意事项,以避免干扰性误差。 * **药典专论解读:** 课程结束后,您将能够熟练处理各类药典专论,并掌握其中的关键技巧。 * **实践操作能力:** 您将能够无障碍地处理标准品、样品和试剂等各类物质。 **课程目标:** 完成本课程后,学员将能够独立完成 HPLC 样品制备和数据计算,能够理解并应用各类计算公式,有效处理和分析质量控制过程中的各类问题,并能够应对各种药典要求。 **致谢与联系:** 感谢您的参与!请在课后对本课程进行评价。如果您有任何疑问,请随时与讲师联系。

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课程详情

Welcome to every one who is interested in pharmaceutical quality control field and thanks for your participation in this course.This course is an advanced course for HPLC samples preparation and calculations, It is highly recommended to quality control analysts with minimum six years experience in quality control laboratory.In this course, I will present thirteen lectures.You will learn details about mobile phase preparation (Buffer solution preparation, pH adjustment, loading the isocratic and gradient injection mode, creating a sequence for assay solutions preparations).Samples preparation for assay test (test solution and standard solution).How to calculate the assay result using the suitable equation with an example.You will know what is the related substances.You will learn how to prepare all solutions needed for related substances test.You will learn types of related substances calculations.You will know the equation of each type.You will see examples for each equation calculation. You will know all the critical precautions that you must follow upon preparing related substances solutions in order to avoid any interfering error.At the end of this course you will be able to deal with any pharmacopeia monograph and understand all tips and tricks, You will be able to deal with all (standards, samples and reagents) practically without any obstructions. Finally, I would be appreciate to evaluate this course after participating and don't hesitate to contact me if you want to understand any information in this course.

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