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所在平台: Udemy |
课程主页: https://www.udemy.com/course/introduction-to-flow-cytometry/
课程评论：没有评论
**课程名称：** 流式细胞术入门 **课程概述：** 本课程旨在帮助初学者或偶尔使用流式细胞术的用户熟悉该领域的多方面知识，从理论视角切入。课程还可作为中级或常用用户的复习，涵盖了跨平台适用的核心概念，包括光散射和荧光的基本原理、流式细胞仪的主要组成部分、多色补偿的实际原则以及流式细胞术中实验对照的作用。 **重要提示：** * 本课程 **不专注于** 任何特定软件或仪器，也 **不旨在替代** 实际操作培训和/或认证课程。 * 课程 **不深入探讨** 样本准备、采集和分析的具体细节，而是提供平台共享的基础知识，为深入学习流式细胞术概念打下基础。 * 讲师拥有15年流式细胞术经验，在学术界和工业界均有任职，对不同规模和应用领域的流式细胞术有深入了解。 * 课程字幕已由讲师审阅和编辑，以确保准确性。 **课程亮点：** * 从理论基础入手，适合新手和有一定经验的用户。 * 涵盖流式细胞术的核心概念和通用原则。 * 提供跨平台的知识，为进一步学习打基础。 * 讲师经验丰富，视角全面。
⭐Average of ALL reviews received, equally weighted: 4.2 stars.⭐This course is designed to help beginner or occasional users of flow cytometry become more familiar with the numerous facets of the field, starting with a theoretical perspective. It may also be useful to intermediate or more frequent operators as a review of the concepts that remain firmly in place across platforms: from the basics of light scattering and fluorescence to the universal main components of flow cytometers, the practical principles of multicolor compensation, and the roles of experimental controls typically used in flow cytometry. Please feel welcome to check out the curriculum/descriptions of the individual videos for a few additional details that may offer a more accurate idea of the scope of the course.VERY IMPORTANT; PLEASE NOTE:The course is NOT focused on any specific software or instruments and is NOT intended to substitute hands-on training and/or certification courses. Instead, it aims to present the elements that constitute a shared ground among platforms and provide a springboard to advanced flow cytometry concepts.The course does NOT go into highly specific details regarding sample preparation, acquisition and analysis. This is due to the variety of possible applications, instruments, reagents and dedicated software, as well as the wide online availability of such protocols (often tailored to particular commercial products) for the most commonly used assays - e.g., cell phenotyping using surface vs. intracellular markers, counting, apoptosis, cell cycle, proliferation, "exotic applications" etc. The instructor is not affiliated with any commercial entity and does not endorse any particular commercially available product (instrument, reagent, software or other) in this course.The instructor has 15 years of experience in flow cytometry, at first in academia as a biomedical researcher, then in the industry as a flow cytometry application scientist. The lectures are built with the awareness that flow cytometry is used for numerous applications, almost all of which rely upon detection of fluorescent molecules. Moreover, in today's "flow" landscape, one-laser instruments coexist with multi-laser analyzers capable of detecting dozens of colors, and several powerful options are available in terms of analysis software. --Captions: The course has captions written from scratch (10 of 21 videos), as well as automatic captions - kindly provided by the platform - that have been reviewed and edited (where necessary) by the instructor.
