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所在平台: Udemy |
课程主页: https://www.udemy.com/course/high-performance-liquid-chromatography-hplc/
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本课程《高效液相色谱法 (HPLC)》旨在全面介绍这种在制药、化妆品、化工、食品和环境等行业广泛应用的技术。 课程涵盖了高效液相色谱法的基本原理、历史、组成部分和应用。具体内容包括： * **色谱法基础**: 介绍色谱法的定义、发展历史、固定相、流动相和分析物的概念。 * **色谱分离原理**: 深入探讨不同分离机制，如亲和、离子交换、溶解度和吸附等。 * **色谱法分类**: 按床、分离机制和相的物理状态对色谱法进行分类，包括柱色谱、纸色谱、薄层色谱、气相色谱和液相色谱等。 * **HPLC系统仪器设计**: 详细解析HPLC系统的各个组成部分，包括溶剂储液器、输液系统（泵）、进样器（自动进样器）、色谱柱、检测器和计算机监控系统。 * **HPLC分析**: 介绍HPLC在定性分析和定量分析中的应用，并提供定量分析实例。 * **HPLC类型**: 重点讲解正相HPLC (NP HPLC)、反相HPLC (RP HPLC)、离子交换色谱 (IEX) 和尺寸排阻色谱 (SEC) 等常见HPLC类型。 * **HPLC描述符和方法开发**: 讨论保留时间、死体积、保留因子、选择性、效率（理论塔板数）、选择性与效率的关系以及分离度等关键参数，为HPLC分析方法的开发奠定基础。 通过本课程的学习，学员将掌握HPLC的核心知识和技术细节，能够理解其在各个领域的关键作用，并为进一步的实验操作和方法开发做好准备。
High Performance Liquid Chromatography (HPLC) techniques are so popular and common in pharma industry, cosmetic industry, chemistry industry, food industry, polymer industry and environment laboratories.High performance liquid chromatography (HPLC) is the crucial and integral analytical tool applied in all stages of drug discovery, development, and commercialization in the pharmaceutical industry, especially. At the each step of drug development, drug substances, ingredients and drug products are controlled and monitored by using HPLC techniques.In this course, chromotography, chromatography principles, types of chromatography, instrumental design of HPLC, HPLC Analysis, Types of HPLC and Descriptors of HPLC have been discussed within all technical details.HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) COURSE SCOPE1 INTRODUCTION 2 INTRODUCTION TO HIGH PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC) 3 CHROMATOGRAPHY 3_1 History of Chromatography 3_2 What is Chromatography? 3_3 What is Stationary Phase? 3_4 What is Mobile Phase? 3_5 What is Analyte? 4 CHROMATOGRAPHY PRINCIPLES 4_1 Chromatographic Seperation 4_2 Chromatography (Affinity & Interaction) 4_3 Chromatography (Solubility & Adsorption) 4_4 Chromatography (Solubility & Polarity) 4_5 Chromatography (Solubility & Hidrophilic / Hidrophobic) 4_6 Let's Categorize Molecules! 5 TYPES OF CHROMATOGRAPHY 5_1 Chromatography Classification Based on Bed (Column Chromatography, Paper Chromatogrphy, Thin Layer Chromatography) 5_2 Chromatography Classification Based on Separation Mechanism (Affinity Chromatography, Ion Exchange Chromatography, Gel Filtration Chromatography) 5_3 Chromatography Classification Based on Physical States of Phases (Gas Chromatography, Liquid Chromatography) 6 INSTRUMENTAL DESIGN OF HPLC SYSTEM 6_1 Instrumental Design of HPLC System_Simple Technical Design 6_2 Instrumental Design of HPLC System_Technical Design 6_3 Instrumental Design of HPLC System_Equipment Compartments 6_4 Instrumental Design of HPLC System_Solvent Reservoir 6_5 Instrumental Design of HPLC System_Solvent Delivery System (Pump) 6_6 Instrumental Design of HPLC System_Sampler Unit & Injector & Autosampler 6_7 Instrumental Design of HPLC System_Column 6_8 Instrumental Design of HPLC System_Dedector 6_9 Instrumental Design of HPLC System_PC Monitor 7 HPLC ANALYSIS 7_1 HPLC in Analytical Chemistry 7_2 Qualitative Analysis 7_3 Quantitative Analysis 7_4 Quantitative Analysis Example 8 TYPES OF HPLC 8_1 Types of HPLC 8_2 Normal Phase HPLC (NP HPLC) 8_3 Reversed Phase HPLC (RP HPLC) 8_4 Ion Exchange Chromatography (IEX) 8_5 Size Exclusion Chromatography (SEC) 9 DESCRIPTORS OF HPLC & ANALYTICAL METHOD DEVELOPMENT9_1 Retention Time 9_2 Void Volume (Dead Volume) 9_3 Retention Factor 9_4 Selectivity 9_5 Efficiency (Teorical Plate Number) 9_6 Selectivity & Efficiency 9_7 Resolution 10 CONCLUSION
