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所在平台: Udemy |
课程主页: https://www.udemy.com/course/gene-delivery-technologies/
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课程名称：基因递送技术 课程概述： 基因治疗是一种独特的技术，利用基因来预防或治愈疾病。这种治疗方法允许医生通过将健康基因插入患者细胞中来治疗疾病，而不是依靠药物或手术。目前，研究人员和医生正在探索多种基因治疗方法，包括：用健康基因替代导致疾病的突变基因；“敲除”或失活错误操作的突变基因；以及引入新基因以保护细胞免受疾病侵害。基因递送系统对于人类遗传疾病的基因治疗至关重要，基因治疗为多种疾病的治疗提供了新希望，如遗传性疾病、病毒感染和癌症。基因递送系统的有效性取决于可调节目标基因递送系统的开放性。 本课程将简要回顾近期在病毒基因递送系统和非病毒基因递送系统方面的进展。我们将讨论基于DNA、RNA的病毒载体，以及肿瘤溶解病毒载体的病毒递送系统。同时，也将介绍物理化学方法，如物理方法和化学方法下的非病毒基因递送系统。在成功的基因递送系统中，将涉及阳离子高分子、聚赖氨酸、多糖及聚乙烯亚胺等不同类型的材料。 主要内容包括： - 病毒传播策略：如慢病毒、腺相关病毒、腺病毒、逆转录病毒、单纯疱疹病毒 - 物理传递策略：裸DNA/质粒DNA、弹道DNA注射、电穿孔、以及水动力和超声波传输技术 - 化学传递技术：无机颗粒（如磷酸钙、金、磁性颗粒）、合成有机颗粒（如聚合物、包封体、脂质体、生物相容性脂质传输） 通过本课程，学员将全面了解基因递送技术的基础知识及其在基因治疗领域的应用。
Gene therapy is a unique technique that uses the gene to prevent or cure any disease. The gene therapy technique could allow doctors to treat a disorder by inserting a gene into a patient's cell instead of using drugs or surgery. Some researchers and physicians are examining a variety of approaches to gene therapy, including i) replacing a disease-causing mutated gene with a healthy gene; ii) "knocking out" or inactivating a misoperated mutated gene, and iii) introducing new genes into cells for protection from any disease.Gene delivery systems are essential for gene therapy of human genetic diseases. Gene therapy is the unique way to use the adjustable gene to treat any disease. Gene therapy is one of the promising treatments for a number of diseases such as inherited disorders, viral infection, and cancers. The beneficial results of gene delivery systems depend on the opening of tunable targeting gene delivery systems. Some successful gene delivery systems have been recently reported for the practical application of gene therapy. Recent advances in viral gene delivery systems and non-viral gene delivery systems for gene therapy are briefly reviewed. Viral gene delivery systems are discussed for viral vectors based on DNA, RNA, and oncolytic viral vectors. Non-viral gene delivery systems have also been treated with physicochemical approaches such as physical methods and chemical methods. Several types of successful gene delivery systems are briefly discussed on the basis of gene delivery systems such as cationic polymers, poly(L-lysine), polysaccharides, and poly(ethyleneimine).•Viral transmission Strategies•Lentiviral, Adeno-associated virus•Adenoviral, Retroviral, Herpes Simplex virus•Physical transfer strategies•Naked/plasmid DNA, Ballistic DNA injection, Electroporation,•Hydrodynamic and ultrasound transmission technologies•Chemical transfer technologies•Inorganic particles (Calcium phosphate, gold, magnetic)•Synthetic organic particles (Polymers, Encapsulation, Liposome, lipid biocompatible transfer)
