Fundamentals Of Flow Cytometry - An Introductory Course

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课程主页: https://www.udemy.com/course/fundamentals-of-flow-cytometry-an-introductory-course/

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课程名称:流式细胞术基础 - 入门课程 概述:流式细胞术是一种现代而强大的生命科学和生物科学工具。当我想到这项技术及其应用时,常常为单个细胞能够如此快速地被分析其物理和化学性质而感到着迷,这一过程可以针对数百万个细胞重复进行。该入门课程将帮助您理解诸如细胞计数免疫表型、流体动力聚焦等术语的含义。此课程非常适合刚开始实验生物学之旅的学生。 课程内容从流式细胞术的基本原理、仪器的功能以及基本参数(如FSC、SSC和荧光信号)的定义开始。在基本讨论后,课程将带您了解流式细胞术的各种重要应用,如活死细胞分析、凋亡分析、细胞周期分析、免疫表型分析和细胞内标记分析等。此课程的一大亮点是,通过虚拟实验以及使用真实的流式细胞术数据和图表,来解释不同的应用。 让我们一起享受这段流式细胞术的入门之旅,将这个重要的分析工具的知识融入到您的学术经历中。

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Flow cytometry is a modern and potent tool of life sciences and biological sciences. Whenever I think about this technology and its applications, I get mesmerized by the fact a single cell can be analyzed so quickly for its physical and chemical properties and this process can be repeated for millions of cells. This introductory course will help you to understand the meanings of words like cytometry immunophenotyping, hydrodynamic focusing and many more. This course is very suitable even for those students who have just begun their journey of experimental biology. This course starts its journey with the discussions on basic principle of flowcytometry, the functioning of instrument and definitions of basic parameters like FSC, SSC and fluorescence signal. After the fundamental discussion this course takes you through various important applications of flowcytometry like live dead cell analysis, apoptosis analysis, cell cycle analysis, immunophenotyping and intracellular marker analysis etc.The best part about this course is that to explain different applications the help of virtual experiments has been taken and original flowcytometry data and graphs have been used. Lets Enjoy this introductory journey of flowcytometry with me and add the knowledge of this important analytical tool in your academic experience.

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