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所在平台: Udemy |
课程主页: https://www.udemy.com/course/from-basics-to-mastery-an-online-hplc-course/
课程评论：没有评论
Coursera HPLC课程：从基础到精通 - 线上分析化学家HPLC培训 本课程专为希望在现代分析实验室中掌握理论知识和实践技能的分析科学家设计。课程将引导学习者从色谱法基础概念（如薄层色谱和柱色谱分离原理）逐步深入，重点讲解HPLC相对于传统柱色谱的优势、HPLC分析流程及关键仪器。 课程内容涵盖HPLC系统的各个组成部分，包括流动相储液罐、泵、进样系统、色谱柱和检测器，并概述了常见的固定相和HPLC技术。 随后，课程将重点介绍方法开发与优化，指导学习者如何利用流程图、信息资源和实践操作来设计稳健的分析方法。优化因素包括色谱柱的材质、粒径、长度和温度，以及流动相的溶剂组成、流速等快速方法改进技巧。 此外，课程还将教授方法验证，强调性能指标和数据收集、色谱图解读、软件参数设置等关键步骤。 课程中穿插了大量的实践环节，包括HPLC系统组装、标准品混合物制备、以及HPLC的实际设置和操作。 最后，课程将提供故障排除和系统维护的必要培训，帮助学员识别、诊断和解决HPLC操作中的常见及疑难问题，并学习对色谱柱进行维护以确保持续的最佳性能。 学完本课程，学员将能够自信地将所学知识应用于实际实验室工作，确保分析结果的准确性和可靠性。
This online HPLC (High-Performance Liquid Chromatography) course, titled From Basics to Mastery: An Online HPLC Course for Analytical Scientists, is meticulously designed to provide a comprehensive, hands-on educational experience for participants seeking to develop both theoretical knowledge and practical skills in modern analytical laboratories. The course is structured to guide learners from foundational concepts to advanced mastery, making it relevant for students and professionals in pharmaceutical chemistry, analytical chemistry, toxicology, quality control, and related fields who aim to achieve proficiency in instrument operation, method development, and data interpretation. Participants will start by learning the basic ideas of chromatography, such as the simple concepts of thin-layer chromatography and the main ways that column chromatography separates substances. The course shows the benefits of HPLC compared to traditional column chromatography, helping learners understand the HPLC analysis process and the important tools used in this method. A close look at the key parts of an HPLC system-like the mobile-phase reservoir, pump, sample injection system, column, and detector-will be provided, along with a summary of common stationary phases and HPLC techniques. As the course progresses, learners engage with method development and optimization, utilizing schematic thinking, information resources, and practical procedures to design robust analytical methods. The course also looks at how to improve methods by considering factors like the column's material, particle size, length, and temperature, as well as the mobile phase's solvent mix, flow rate, and other techniques for quick method improvement. The course also teaches how to check if methods are working well, focusing on important performance factors and the steps needed to validate an analytical method, which includes collecting data, understanding chromatograms, and setting up software parameters. The course integrates practical sessions throughout, emphasizing HPLC system assembly, standard mix preparation, and hands-on HPLC setup and operation. Finally, the course provides essential training in troubleshooting and system maintenance, equipping participants with the skills to identify, diagnose, and resolve both common and serious HPLC problems, as well as maintain columns for optimal performance. By the end of the course, participants will be well-prepared to apply their knowledge in real-world laboratory settings, ensuring accurate and reliable analytical results.
