|
所在平台: Udemy |
课程主页: https://www.udemy.com/course/differential-expression-analysis-using-edger/
课程评论：没有评论
**课程名称：** 使用edgeR进行差异表达分析 **课程概述：** 本课程由生物信息学家George指导，旨在教授如何使用R语言和edgeR工具包对RNA测序数据进行差异表达分析。课程将以公开的银屑病关节炎数据集为例，比较银屑病关节炎患者与健康对照组的基因表达差异，识别失调基因。 **课程内容：** * **数据准备与清洗：** 对RNA测序数据进行初步处理和清洗。 * **探索性分析：** 对数据进行探索性分析，了解数据特征。 * **数据标准化：** 对数据进行标准化处理，消除批次效应等。 * **差异表达分析：** 使用edgeR找出在不同样本组之间表达差异显著的基因。 * **结果可视化：** 绘制火山图和热图，直观展示差异表达基因。 * **结果文档化：** 保存分析结果，确保其可重复性。 **目标学员：** * 对RNA测序数据分析感兴趣，特别是希望学习edgeR应用的初学者。 * 具备基本的R编程经验。 * 能够安装R语言环境到个人电脑。 **学习目标：** * 掌握使用edgeR进行RNA测序差异表达分析的整个流程。 * 能够独立完成从数据准备到结果可视化的分析。 * 了解现代RNA测序数据分析的常用方法。
Hello everyone! My name is George and I am a bioinformatician. I am here to guide you through an analysis of RNA sequencing data using edgeR. This is a differential expression analysis where we compare samples from Psoriatic Arthritis patients to Healthy controls in order to identify deregulated genes. It is a very simple and common experimental design that is an industry-standard nowadays and a great tool to have at your disposal. For this course, you need to have some basic R programming experience and you need to have a computer with R language installed. This course is intended for people aiming to dive into the world of RNA sequencing data analysis and have not had the chance to analyse data using edgeR before.We will use the publicly available Psoriatic Arthritis dataset and perform some data cleaning, preparation, exploratory analysis, data normalization and subsequently reach our desired goal which is the differential expression analysis. Finally, we will visualize the results by creating a volcano plot and a heatmap of the top differentially expressed genes and we will save any information needed for this analysis to be documented in a reproducible way.If you fulfil the above-mentioned criteria, tag along and let's explore the basics of differential expression using edgeR!
