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所在平台: Udemy |
课程主页: https://www.udemy.com/course/crispr-genetic-editing-methods/
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**课程名称：CRISPR基因编辑技术在靶向疾病中的应用** **课程概述：** 本课程深入探讨了CRISPR基因编辑技术的原理、发展及其在靶向疾病治疗中的巨大潜力。CRISPR/Cas系统作为一种源于原核生物免疫防御机制的强大工具，已在基因工程领域掀起革命，展现出前所未有的灵活性、易用性和成本效益，远超以往的技术。 课程将从CRISPR/Cas系统的基本概念入手，阐述其在细菌抵御病毒入侵中的关键作用，以及如何被巧妙地应用于基因工程。特别是CRISPR/Cas9系统的出现，通过导向RNA（gRNA）精确引导Cas9核酸内切酶在特定位点引发DNA双链断裂，再通过同源重组修复（HDR）实现基因编辑。相比于ZFNs和TALENs等技术，CRISPR/Cas9的高灵敏度、易于调控、成本低廉和时间效率使其在基因工程应用中脱颖而出，尤其适用于高通量基因编辑。 我们正处于基因工程的黄金时代，CRISPR/Cas9等技术的成熟为临床应用奠定了坚实基础。当前研究致力于提高技术的靶向精确度、特异性和疗效，以期在临床上广泛应用。Addgene致力于推动基因工程技术的普及，使全球研究人员都能便捷地使用这些强大的工具。本课程将全面解析CRISPR技术，帮助您掌握这一前沿领域。 **课程内容模块：** i. CRISPR-Cas简介 ii. CRISPR-Cas系统详解 iii. CRISPR基因治疗应用 iv. CRISPR项目规划 v. 导向RNA设计与Benchling软件应用 vi. 基因编辑的验证方法
The CRISPR revolution has begun and shows no signs of slowing down. This system, which is the key to prokaryotic adaptive immunity, has proven to be particularly under the influence of genome engineering. CRISPR/Cas provides flexibility as well as easily amplifying the signal and regulating the signal level, which is beyond the capabilities of previous systems. Prokaryotes have long exploited CRISPR/Cas as a powerful defense strategy against virus-derived invaders, and eventually, this system turns out to be useful only for research applications.Just when we thought that genome engineering could not get any further, the CRISPR (clustered regularly spaced short palindromic repeats)/Cas9 system emerged. It is an important component of the bacterial immune system, allowing bacteria to recognize and destroy phages. In genome engineering applications, guide RNA (gRNA) sequence homology targets the Cas9 endonuclease to a specific locus-where the double helix is broken. Like ZFNs and TALENs, CRISPR/Cas9 uses HDR, but using RNA to determine editing makes the system more sensitive and malleable, as well as less expensive and time-consuming. For this reason, CRISPR/Cas9 has proven invaluable for high-throughput genome engineering.With a variety of robust and effective genome engineering methods at our fingertips, we have entered the Golden Age of genome engineering. Current studies have focused on developing these techniques in the hope that they will be clinically useful to provide desired target loci (or loci), high specificity, and efficacy. The most exciting thing we've found at Addgene is the democratization of genome engineering that has enabled and will continue to enable researchers around the world to use these tools. To learn all about CRISPR, you must complete the course.CRISPR/Cas Gene Editing Training Programi. What is CRISPR-Cas?ii. What are CRISPR-Cas Systems?iii. CRISPR Genetic Therapy Applicationsiv. Planning Your CRISPR Projectv. Guide RNA Design & Benchlingvi. Confirmation of your Gene Editing
