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所在平台: Udemy |
课程主页: https://www.udemy.com/course/crispr-cas-system-applications-in-gene-editing-and-beyond/
课程评论：没有评论
课程名称：CRISPR Cas系统：基因编辑及其应用 课程概述：基因编辑技术CRISPR-Cas系统被誉为"十年来最大的技术之一"，在医学、农业等多个领域展现出广泛应用。本课程深入探讨基因编辑的细节，探索其在治疗镰状细胞病、囊性纤维化等遗传疾病中的潜力，以及在工程作物和消灭传播疟疾的蚊子等应用中的前景。您将学习CRISPR作为基因编辑工具的高级知识，与其他基因编辑工具如锌指核酸酶、TALENs等的比较、CRISPR-Cas系统的组成以及在医疗、农业等行业的不同应用。 CRISPR系统的魅力在于其应用超越了基因编辑本身。本课程将学习科学家如何修改CRISPR系统，以可逆的方式激活或沉默基因，并以此前所未有的方式编辑表观基因组，这将为新的治疗选择和人类病因学的理解打开新的大门。此外，本课程还将介绍如何将荧光蛋白附加到CRISPR-Cas的组件上，以检测和定位染色体上的特定DNA序列，这对可视化基因组的三维结构及识别影响正常和病态细胞功能的各种染色体异常（如缺失、重复和易位）十分有用。 课程中还会讨论研究人员如何利用CRISPR技术诊断包括COVID-19在内的传染病，并讨论CRISPR-Cas系统的局限性与挑战。此外，课程将详细讨论CRISPR介导的精确基因编辑技术，如基础编辑和精简编辑，这些技术可以革命性地改变遗传疾病的治疗，同时降低CRISPR-Cas系统的负面影响。本课程还将讲解多种Cas9核酸酶变体的工程及其重要性。 本课程是医学学生、医生以及生物技术、生物学、免疫学、遗传学、分子生物学、细胞生物学和生物信息学等学科学生的宝贵资源。课程中以简单易懂的动画和图形形式介绍最具挑战性的概念。
Gene editing with the CRISPR-Cas system has been called "one of the biggest technologies of the decade" for its applications in medicine, agriculture, and much more. And in this course we will delve deep into the details of gene editing and why it is being seen as a promising strategy for the treatment of numerous genetic diseases like sickle cell disease, cystic fibrosis, etc to the generation of engineered crops, wiping out of malaria causing mosquitoes and others. You will get advanced learning of how CRISPR functions as a gene-editing tool, its comparison to other gene editing tools like Zinc Finger Nucleases, TALENs, etc, components of the CRISPR-Cas system, and the different ways in which CRISPR can be applied to healthcare, agriculture, and many other industries.The beauty of the CRISPR system is that its applications extend much further beyond just gene editing.For instance, the CRISPR-Cas system can be used as a powerful tool to regulate gene expression. In this course, we will learn how scientists have been modifying the CRISPR system to reversibly activate or silence genes and edit the epigenome in previously unimaginable ways. This can open the doors to new treatment options and a better understanding of human pathogenesis.This course will cover how fluorescent proteins can be attached to the components of CRISPR-Cas to detect and locate the positions of specific DNA sequences on the chromosomes. This, in turn, can be useful to visualize the 3D architecture of the genome or to know various chromosomal abnormalities, including deletions, duplications, and translocations that influence cell function in normal and diseased states.Additionally, we will also discuss, how using CRISPR, the researchers have expanded chromatin immunoprecipitation or ChIP to identify proteins associated with a particular genomic sequence.Further in this course, we will talk about how scientists have harnessed the CRISPR-Cas technology for diagnosing infectious diseases including COVID-19.The limitations and challenges of the CRISPR-Cas system have also been discussed. Additionally, this course will provide a detailed discussion on CRISPR-mediated precise gene editing techniques like prime editing and base editing, that can revolutionize the treatment of genetic diseases while limiting the negative effects of the CRISPR-Cas system. For precise gene editing and non-gene editing applications, several variants of Cas9 nuclease have been engineered. In this course, you will get a thorough understanding of these variants, their engineering, and their significance.This course is an invaluable resource for medical students, doctors, and students of disciplines like biotechnology, biology, immunology, genetics, molecular biology, cell biology, and bioinformatics. In the course, the most challenging concepts are presented in a simple and palatable format using animations and graphics.
