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所在平台: Udemy |
课程主页: https://www.udemy.com/course/cloning-dry-lab/
课程评论：没有评论
课程名称：克隆干实验室 概述：本课程旨在通过生物信息学和计算生物学的手段，研究基因克隆，以辅助实验室中的DNA克隆实施。课程内容侧重于使用简单易操作且不耗时的软件，帮助您深入了解DNA克隆的细节。克隆载体是DNA克隆过程成功的关键元素，它承载着目标基因并将其转移至重要位点。为了实施克隆，您需要了解限制酶的位点，聚焦于特定位置以验证插入到克隆载体中的成功性。克隆载体和目标基因都必须在相同的限制位点上使用相同的限制酶进行切割，以形成黏性末端，从而便于后续连接。切割必须在分子标记的中间进行，以便验证过程的成功，这需要您不仅了解限制位点的位置，还需掌握分子标记和基因表达所需的启动子的位置。为了准确识别启动子位置，您需要对该区域进行放大，而放大的前提是您必须具备引物，因此Snap Gene软件也提供了设计引物所需的工具。 无大纲。
The use of bioinformatics and computational biology to study gene cloning has been an auxiliary method used to help implement the DNA cloning in the lab, it facilitates the tools needed to conduct the process efficiently and accurately.the course provides easily handled and non-time consuming software, it gives you the privilege to dive into the deeps of DNA cloning details, cloning vectors are the key elements for the success of the DNA cloning process as it carries the gene of interest and transfer it to the site of importance. So, in order to conduct the process you have to get the knowledge of the locations of restriction sites and restriction enzymes focusing on the certain locations for the purpose of testing the success of the insertion into the cloning vector. The cloning vector and the gene of interest must be both cut at the same restriction sites using the same restriction enzymes in order to make sticky ends to facilitate their ligation later on, the cut must be done in the middle of a molecular marker in order to test the success of the process and this can be easily done by knowing not only the locations of the restriction sites but also the locations of molecular markers and locations of the promoters for gene expression later on.In order to know the exact position of promoter this region must be amplified and in order to do that you have to possess primers, that is why Snap gene software also provides you with tools to design a primer.
