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所在平台: Udemy |
课程主页: https://www.udemy.com/course/bioinformatics-mastery-genome-engineering-using-crispr-cas9/
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### 生物信息学精通：CRISPR-Cas9基因组工程 本课程深入探讨基因组工程，特别是利用CRISPR-Cas9技术。基因组工程，又称基因编辑，是指在细胞或生物体的DNA中进行精确、可控的改造，包括插入、删除、修改或替换特定的DNA序列。 CRISPR-Cas9系统作为第三代基因组编辑工具，相较于前两代（ZFNs和TALENs），具有更简便、更快速、更经济、更精确的优势，因此在基因组工程中得到了广泛应用。CRISPR（规律间隔成簇短回文重复序列）负责识别和定位DNA，而Cas9是一种核酸酶，负责切割DNA。 CRISPR-Cas9技术最初发现于细菌和古细菌的RNA介导的适应性免疫系统中，能够通过整合病毒DNA片段来抵抗噬菌体侵染。 该技术的应用前景广泛，包括治疗遗传性疾病（通过纠正致病基因）、构建人类疾病的细胞和动物模型、改进生物技术和食品生产，以及深入研究基因功能和调控元件等。 本课程将理论与实践相结合，首先介绍基因组工程和CRISPR-Cas9的基本原理，包括： * **基因编辑基础：** NHEJ途径、HDR途径、限制性内切酶。 * **传统与新型基因编辑工具：** ZFNs、TALENs、CRISPR-Cas9。 * **CRISPR-Cas9基因组工程应用：** mRNA、外显子序列检索。 * **重要的生物信息学工具：** * **gRNA设计工具：** CRISPRdirect, WU-CRISPR, CAS designer, GT-Scan, Off-Spotter, Guides, GPP sgRNA designer, CRISPR-Cas9 gRNA design checker, CHOPCHOP, CRISPRON。 * **脱靶预测工具：** (具体名称未在syllabus中明确列出，但提及了此类工具的重要性) 通过本课程的学习，学员将掌握基因组工程及其CRISPR-Cas9技术的理论知识，并学会利用相关的生物信息学工具进行实际分析，显著拓展对基因编辑领域的认知。
Genome engineering or gene editing is a way of making specific changes to the DNA of a cell or organism in a controlled way. It is a type of genetic engineering in which DNA is inserted, deleted, modified, or replaced in the genome of a living organism.During the genome editing process, a type of enzyme called "engineered nuclease" cuts the genome in a specific place. When this is repaired by the cell, a change or an edit is made at the sequence, leading to a change in characteristics of a cell or an organism.There are 3 generations of nucleases for genome editing: (1) Zing Finger Nucleases (ZFNs), (2) TALENs (Transcription activator-like effector nuclease), (3) CRISPR/Cas9.CRISPR/Cas9 is simpler, faster, cheaper, and more accurate than older genome editing methods and thus, it is most commonly used in the genome engineering process. CRISPR stands for Cluster Random Interspaced Short Palindromic Repeats. It is the DNA-targeting part of the system which consists of an RNA molecule, or ‘guide' designed to bind to specific DNA bases through complementary base-paring. Cas9 is the CRISPR-associated protein 9 and is the nuclease part that cuts the DNA.The CRISPR/Cas9 system was initially identified as the RNA-based adaptive immune system in bacteria and archaea. The native CRISPR system confers resistance to viruses by incorporating "short repeats" of the viral DNA into the bacterial genome.CRISPR Cas9 technique can be used to treat many hereditary disorders by modifying the mutated genotypes to a normal phenotype. Other applications include the creation of cellular and animal models of human disease, Improvements in biotechnology and food production, the study of the functions of genes and gene regulatory elements, and many others.This course introduces you to the world of genome engineering and CRISPR/Cas9. Throughout the course, we will cover essential theory knowledge to understand the genome engineering process and then will start with various bioinformatics tools like guide RNA design tools, off-target prediction tools and many others used during CRISPR/Cas9 mediated gene editing.The detailed course structure includes;Genome EditingNHEJ PathwayHDR PathwayRestriction EnzymesZinc Finger NucleasesTALENsCRISPR/Cas9Genome Engineering using CRISPR Cas9Retrieval of mRNA of a GeneCRISPRdirectRetrieval of Exon SequenceWU-CRISPRCAS designerGT-ScanOff-SpotterGuidesGPP sg RNA designerCRISPR Cas9 gRNA design checkerCHOP CHOPCRISPORThis course is a unique blend of theory and practical, where you will learn basic theory and then perform practical analysis of Genome engineering and CRISPR/Cas9 concepts. We assure you that after taking this course, your perspective will be very different for Gene editing. So, sign up for the course and see how fun, exciting, and rewarding the genome engineering tools are. We hope this course will be worth your money and time.
