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课程名称:生物化学:临床方面 课程概述:生物化学可以被视为对生命组成成分及其组合的研究,这一领域的历史可以追溯到古希腊时期。然而,生物化学作为一门具体的科学学科始于19世纪或更早。1833年,安瑟尔梅·佩恩发现的第一个酶——淀粉酶,有人认为这是生物化学的开端;而爱德华·布赫纳于1897年首次在无细胞提取物中演示复杂的生化过程——酒精发酵,亦被视为生物化学的起始。其他早期的生化先驱,如尤斯图斯·冯·吕比希的《动物化学》一书和埃米尔·费舍尔对蛋白质化学的研究,对现代生物化学的发展也起到了重要作用。 “生物化学”一词源自生物学和化学的结合,1877年,费利克斯·霍普-塞勒在《生理化学杂志》第一期前言中首次使用该术语,鼓励设立专门的研究机构。而德国化学家卡尔·纽伯格被认为在1903年正式创造了这个词,尽管也有人将其归功于弗朗茨·霍夫梅斯特。 生物化学是研究与生命体内及相关的化学过程的学科,是化学和生物学的一个子学科,主要分为结构生物学、酶学和代谢学三个领域。过去几十年,生物化学在这三个领域成功地解释了生命过程,几乎所有生命科学领域都通过生物化学的方法论和研究得到揭示和发展。 生物化学的重点是理解生物分子的化学基础,如何促成细胞内及细胞之间发生的各种过程,这对于理解组织、器官以及生物体的结构和功能具有重要意义。生物化学与分子生物学密切相关,后者关注生物现象的分子机制。生物化学涉及生物大分子的结构、结合、功能和相互作用,如蛋白质、核酸、碳水化合物和脂质,这些大分子构成细胞的结构并执行生命相关的许多功能。细胞的化学反应还依赖于小分子和离子的反应,这些可以是无机的(如水和金属离子)或有机的(如用于合成蛋白质的氨基酸)。细胞从环境中获取能量的机制被称为代谢。生物化学的发现主要应用于医学领域,生物化学家研究疾病的原因和治疗方法。
At its most comprehensive definition, biochemistry can be seen as a study of the components and composition of living things and how they come together to become life. In this sense, the history of biochemistry may therefore go back as far as the ancient Greeks. However, biochemistry as a specific scientific discipline began sometime in the 19th century, or a little earlier, depending on which aspect of biochemistry is being focused on. Some argued that the beginning of biochemistry may have been the discovery of the first enzyme, diastase (now called amylase), in 1833 by Anselme Payen, while others considered Eduard Buchner's first demonstration of a complex biochemical process alcoholic fermentation in cell-free extracts in 1897 to be the birth of biochemistry. Some might also point as its beginning to the influential 1842 work by Justus von Liebig, Animal chemistry, or, Organic chemistry in its applications to physiology and pathology, which presented a chemical theory of metabolism, or even earlier to the 18th century studies on fermentation and respiration by Antoine Lavoisier. Many other pioneers in the field who helped to uncover the layers of complexity of biochemistry have been proclaimed founders of modern biochemistry. Emil Fischer, who studied the chemistry of proteins, and F. Gowland Hopkins, who studied enzymes and the dynamic nature of biochemistry, represent two examples of early biochemists.The term "biochemistry" itself is derived from a combination of biology and chemistry. In 1877, Felix Hoppe-Seyler used the term (biochemie in German) as a synonym for physiological chemistry in the foreword to the first issue of Zeitschrift für Physiologische Chemie (Journal of Physiological Chemistry) where he argued for the setting up of institutes dedicated to this field of study. The German chemist Carl Neuberg however is often cited to have coined the word in 1903, while some credited it to Franz Hofmeister.Biochemistry or biological chemistry, is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells, in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenomena. Much of biochemistry deals with the structures, bonding, functions, and interactions of biological macromolecules, such as proteins, nucleic acids, carbohydrates, and lipids. They provide the structure of cells and perform many of the functions associated with life.[6] The chemistry of the cell also depends upon the reactions of small molecules and ions. These can be inorganic (for example, water and metal ions) or organic (for example, the amino acids, which are used to synthesize proteins). The mechanisms used by cells to harness energy from their environment via chemical reactions are known as metabolism. The findings of biochemistry are applied primarily in medicine wherein biochemists investigate the causes and cures of diseases.