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所在平台: Udemy |
课程主页: https://www.udemy.com/course/analytical-techniques/
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课程名称：分析技术 课程概述：生物化学中常用的分析方法至关重要，掌握这些方法不仅是必要的，更是必不可少的。这是因为这些方法和实验构成了理论的坚实基础，是了解该学科的主要知识来源。本课程涵盖了广泛的技术、原理、实验细节等内容，涉及离心分离（亚细胞分馏等）、基于流体力学特性的分离技术、色谱和电泳技术、高效液相色谱（HPLC）、纯化程序、酶联免疫吸附测定（ELISA）及其他技术。这些技术在临床生物化学、分子诊断、聚合酶链反应（PCR）、放射免疫分析（RIA）、正电子发射计算机断层扫描（PET）以及其他临床诊断技术中有广泛应用，同时也适用于生物医学研究。 课程内容包括： - 水溶液、酸、碱、缓冲系统及pH计 - 比色法和紫外-可见光吸收光谱法 - 流体力学技术的介绍 - 离心分离：原理与方法 - 密度梯度离心：速率区分法与等密度法 - 亚细胞分馏的差速离心 - 凝胶过滤：原理、方法与应用 - 平面色谱：原理与应用 - 离子交换色谱、亲和色谱 - 气相色谱、高效液相色谱（HPLC）、蛋白质估算技术 - 电子显微镜（TEM和SEM） - 荧光显微镜：活细胞成像应用 - 免疫组化（IHC）与免疫荧光（IF） - 流式细胞术：理论与概念，以及在生物学与医学中的应用 - 光学显微镜与共聚焦显微镜 - SDS-PAGE、西方印迹技术、二维电泳及差异凝胶电泳（DIGE） - 质谱原理 该课程深入介绍了这些关键分析技术的理论与应用，旨在帮助学生建立坚实的生物化学分析基础。
The analytical methods that are frequently employed in biochemistry are crucial, and mastering them is not only necessary but also vital. This is due to the fact that the solid foundation provided by the methods and experiments upon which the theories are based is the primary source of knowledge on the subject. This course covers a wide range of techniques, their principles, experimental details, etc. These include centrifugation (sub-cellular fractionation, etc.), separation techniques based on hydrodynamic properties, chromatographic and electrophortic techniques, HPLC, purification procedures, ELISA, and other techniques. Use in clinical biochemistry, molecular diagnostics, PCR, RIA, PET, and other clinical diagnostic techniques; also applied in biomedical research. This Course includes: Aqueous solutions , Acids, Bases, buffers systems and pH meter, Colorimetry and UV-VIS absorption spectroscopy Introduction to Hydrodynamic Techniques. Centrifugation: Principles & Methodology Density Gradient centrifugation: Rate Zonal and isopycnic Differential centrifugation for Sub-cellular Fractionation. Gel filtration: Principle, Methodology & Applications Planar Chromatography: Principles and Applications. Ion-exchange chromatography, Affinity chromatography. Gas chromatography, HPLC, Protein Estimation Techniques. Electron Microscopy, TEM and SEM Fluorescence Microscopy: Application to live cell imaging. IHC and IF, Flow cytometry: Theory and concept Flow cytometry:Application in biology and medicine. Light Microscopy and Confocal Microscopy, SDS-PAGE. Western Blotting Techniques, 2D Electrophoresis and DIGE, Principles of Mass spectrometry
