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所在平台: Udemy |
课程主页: https://www.udemy.com/course/all-kinds-of-plant-tissue-culture-concepts/
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**课程名称：** 植物组织培养概念全集 **课程概述：** 本课程旨在全面介绍植物组织培养的核心概念。我们将从植物组织培养的奠基人——奥地利植物学家Gottlieb Haberlandt（1854-1945）讲起，他于1902年提出的"全能性"（Totipotentiality）理论是整个领域的基础。 课程将深入讲解 **外植体（Explant）** 的概念，即具有再生潜力的植物部分，能够发育成完整植株。我们将分步骤学习 **愈伤组织培养（Callus culture）** 的过程，包括： * **外植体的选择**：可从植物的任何部分选取分化组织作为外植体，外植体的细胞分裂和增殖能力会影响培养结果。 * **表面灭菌**：去除外植体表面的微生物。 * **影响愈伤组织培养的因素**：重点介绍物理因素和生长调节剂（如生长素、细胞分裂素）的作用。 * **悬浮培养（Suspension culture）**：从愈伤组织发展而来的细胞培养。 课程还将详细探讨 **愈伤组织** 的特性：它是一种未分化、无组织的植物细胞团，通常在分化组织或器官的伤口处形成。 我们将进一步介绍 **培养基（Media）** 的主要类型，如MS培养基、B5培养基、N6培养基和Niches培养基，并详细解析培养基的组成要素，包括： * **无机营养**：常量营养元素和微量营养元素，以及它们之间的区别。 * **碳/能源**：植物生长所需的碳源。 * **有机补充物**：维生素、氨基酸等。 * **其他有机补充物**：如活性炭、抗生素。 * **生长调节剂**：生长素（Auxins）、细胞分裂素（Cytokinin）、赤霉素（Gibberellins）等，及其在植物生长促进、生长功能和应用方面的作用，以及植物生长抑制剂（如ABA）的应用和效应，乙烯（Ethaline）的用途。 * **固体化剂**：如明胶。 * **培养基pH值**：合适的pH值、选择和灭菌。 最后，课程会提及 **植物组织培养的分类**，并展望其 **商业应用**。 *请注意：课时安排将与直播会话同步进行。*
Who is the father of tissue culture?Gottlieb HaberlandtGottlieb Haberlandt is known as the father of plant tissue culture.Gottlieb Haberlandt (28 November 1854 to 30 January 1945) was an Austrian botanist. His original idea presented in 1902 was called Totipotentiality.ExplantAn explant is the part of a plant which has got the regeneration potential and is capable to give rise to the whole plant.Step 1 Explant for callus cultureStep 2 Surface sterilizationStep 3 Factors affecting callus culture Physical factors Growth regulatorsStep 4 Suspension culture from callusCallus culture callus is the undifferentiated and unorganized mass of plant cellsIt is basically a tumor tissue which usually forms on wounds of differentiated tissues or organsCallus formation in vivo is frequently observed as a result of wounds at edges of stems or rootsExplant for callus culture The explant for callus culture may be the differentiated tissue from any part of the plant The explant for callus culture may be the differentiated tissue from any part of the plant The selected explant tissues may be at different stages of cell division cell proliferation and organization into different distinct specialized structuresIf the explant used possesses meristematic cells then the cell division and multiplication will be rapidFactors affecting callus culture Many factors are known to influence callus formation in invitro culturesMS medium most commonly used physical factors and growth factorsRest will continue with live session.Major types of MediaMS mediaB5 mediaN6 MediaNiches mediaClassification of Plant tissue cultureinorganic nutrientsCarbon Energy sourcesMacro nutrientsMicro nutrientsDifference between macro and micro nutrientsOrganic supplementsVitaminsAmino acidsother organic supplementsactivated charcoalAntibioticsGrowth regulatorsAuxinsCytokininGibberellinsPlant growth promotersPlant growth functionsPlant growth applicationscommercial usageplant growth inhibitorsABAapplications of ABAeffects of ABAEthaline usesSolidifying agentsGelatinPH of MediumSuitable, selection and sterilization of mediumOverview
