Directed Evolution: Engineering Biocatalysts

开始时间: 04/22/2022 持续时间: 未知

所在平台: MIT公开课

课程类别: 生物与生命科学

大学或机构: ocw

课程主页: http://ocw.mit.edu/courses/biology/7-344-directed-evolution-engineering-biocatalysts-spring-2008

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课程简介

Directed evolution has been used to produce enzymes with many unique properties. The technique of directed evolution comprises two essential steps: mutagenesis of the gene encoding the enzyme to produce a library of variants, and selection of a particular variant based on its desirable catalytic properties. In this course we will examine what kinds of enzymes are worth evolving and the strategies used for library generation and enzyme selection. We will focus on those enzymes that are used in the synthesis of drugs and in biotechnological applications.

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