Experimental Methods in Systems Biology

开始时间: 04/22/2022 持续时间: 8 weeks

所在平台: CourseraArchive 课程类别: 生物与生命科学 大学或机构: Icahn School of Medicine at Mount Sinai(西奈山伊坎医学院) 授课老师： Marc Birtwistle

课程主页: https://www.coursera.org/course/expmethods

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课程详情

This course will cover techniques in genomics, proteomics, metabolomics, genome wide functional screens, chemical library screens and high content imaging. Each approach will be introduced in the context of the biological questions the approach is used to address. There will be a discussion of the strengths and limitations of the technology. The experiments will be described from start to finish using videos. Treatment protocols and sample preparation will be described. Experts who run cores will describe the machinery and technologies used for these experiments. The raw data is obtained and the methods of statistical analyses will be discussed. Descriptions of experimental methods underlying dynamical models will be described. Genome wide screen for functional assays, including high content imaging and chemical library screening for drug discovery will also be introduced. The course will conclude with the description of publicly available databases.

课程大纲

Topics covered include:

• Introduction to High Throughput Experimentation - Strengths and Weaknesses
  
• Genomics - DNA Sequencing | Identifying Mutations | Identifying SNPs | Next Generation Sequencing | mRNA Seq
• Microarray Technologies 
  
• DNA Methylation by Sequencing and Chips
  
• Proteomics
  
• Phosphoproteomics by MS
  
• Reverse Phase Protein Arrays
  
• Microwesterns
  
• Quantification of Protein Levels within Cells
  
• Measuring and Estimating Kinetic Parameters
  
• Metabolomics
  
• Genomic-wide Functional Screens
  
• High Content Imaging
  
• Chemical Library Screening
  
• Experimental Systems Biology Databases

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