Metagenomics applied to surveillance of pathogens and antimicrobial resistance

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课程简介

Technical University of Denmark (DTU)

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In this module, you will be introduced to metagenomics, some of the considerations and controls that need to be in place in a metagenomics study, and to the topic of antimicrobial resistance. You will also learn about: 1) Sampling and sample handling - the considerations behind a sampling plan, how to perform sampling in practice, and how sample storage can affect metagenomics results; 2) DNA and RNA extraction methods - both for bacterial and viral microorganisms; 3) Sequencing - from library preparation to the basics of different sequencing technologies.

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The field of metagenomics and whole community sequencing is a promising area to unravel the content of microbial communities and their relationship to disease and antimicrobial resistance in the human population. Bioinformatic tools are extremely important for making sense out of metagenomics data, by estimating the presence of pathogens and antimicrobial resistance determinants in complex samples. Combined with relevant explanatory data, metagenomics is a powerful tool for surveillance. In this course, we teach about the potential of metagenomics for surveillance and give the learners an overview of the steps and considerations in a metagenomics study. After this course, the learners will know: - the difference between the concepts of metagenomics and other microbial genomics - the need to use controls in different steps of a metagenomics study - the advantages of metagenomics for the surveillance of antimicrobial resistance - how sampling design, sample size, sample material and sample handling influence the outcome of a metagenomics study - sample processing for bacterial and viral metagenomics - different sequencing platforms and their possibilities regarding metagenomics - the steps involved in a general metagenomics study, including quality control, mapping to different databases, and read count analysis - the principles behind various tools available for analysis of metagenomics data - how to interpret read classification results - the need for epidemiology in surveillance - the concept of global and integrated surveillance - the challenges for the use of metagenomics in surveillance - the potential of metagenomics for surveillance We look forward to welcoming you !

元基因组学应用于病原体和抗菌素耐药性的监测:宏基因组学和整个社区测序领域是一个有希望的领域,可以揭示微生物群落的含量及其与人群疾病和抗菌素耐药性的关系。通过估计复杂样品中病原体和抗菌素耐药性决定因素的存在,生物信息学工具对于从宏基因组学数据中弄清意义非常重要。结合相关的解释性数据,宏基因组学是一种强大的监视工具。 在本课程中,我们讲授了宏基因组学在监视方面的潜力,并为学习者提供了宏基因组学研究中的步骤和注意事项的概述。学习完本课程后,学习者将知道: -宏基因组学和其他微生物基因组学的概念之间的区别 -在宏基因组学研究的不同步骤中使用控件的需求 -宏基因组学在监测抗菌素耐药性方面的优势 -抽样设计,样本大小,样本材料和样本处理如何影响宏基因组学研究的结果 -细菌和病毒宏基因组学的样品处理 -不同的测序平台及其在宏基因组学方面的可能性 -一般宏基因组学研究涉及的步骤,包括质量控制,映射到不同的数据库以及读取计数分析 -可用于宏基因组学数据分析的各种工具背后的原理 -如何解释阅读的分类结果 -监测中需要流行病学 -全局和集成监视的概念 -在监测中使用宏基因组学的挑战 -宏基因组学的监测潜力 我们期待着您的光临!

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