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所在平台: Coursera |
课程主页: https://www.coursera.org/learn/extracellular-vesicles
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课程名称：细胞外囊泡基础 课程概述：本课程旨在提供关于细胞外囊泡（EV）的基本知识，细胞外囊泡是一个包括外泌体、微囊泡、微粒、外囊泡、肿瘤囊泡、前列腺囊泡等的通用术语。课程内容涵盖EV的历史、命名法、生成、生物学载荷及其释放与摄取机制、收集与处理前的隔离方法，以及不同的隔离、表征和定量技术。 该课程分为五个模块。模块1介绍该领域，包括EV的命名法和历史。模块2侧重于EV的生成、释放与摄取机制，以及不同的EV载荷（如RNA、蛋白质、脂质）。模块3专注于细胞培养基和如血液、母乳、脑脊液及尿液等体液的收集和处理，以便于隔离EV。模块4和模块5则展示不同的隔离方法和表征/定量技术，包括差速超离心、分子筛层析、密度梯度、基于试剂盒的沉淀、电子显微镜（EM）、冷冻透射电子显微镜（cryo-TEM）、流式细胞术、原子力显微镜和纳米粒子追踪分析等。 推荐的先修课程为大学一年级的生物学和生物化学。完成课程后，您应能够： + 讨论细胞外囊泡的命名法和亚组。 + 描述细胞外囊泡的RNA、蛋白质和脂质内容。 + 描述最常见的隔离与表征技术的基本概念，以及这些技术在EV领域的应用。 + 陈述最常见的细胞外囊泡隔离与表征技术的优缺点。 + 解释在不同体液中收集和隔离EV时的重要考虑因素。 + 描述细胞外囊泡的释放和摄取机制。 所有讲座均用英语进行。 每个模块后将进行一次考试，考试形式为多项选择题。 本课程由国际细胞外囊泡学会（ISEV）、加州大学尔湾分校（美国）、哥德堡大学（瑞典）和浦项科技大学（韩国）联合组织。
Name:Introduction to the Course and the Field of Extracellular Vesicles
Description:In this module, we will introduce the course and the field of extracellular vesicles. We will discuss the nomenclature and the history of extracellular vesicles as well as the organisms and places that have been shown to release/contain extracellular vesicles.
Name:Biogenesis, Cargo and Uptake of Extracellular Vesicles
Description:In this module, we will focus on the biogenesis and release of extracellular vesicles as well as the uptake mechanisms of extracellular vesicles when they are encountered by a recipient cell. This module also covers the different RNA, protein and lipid types present in extracellular vesicles as well as a brief overview what the functions of these molecules are. Furthermore, the techniques that are commonly used to detect these molecules as well as databases that can be useful to use when analyzing the cargo of extracellular vesicles will be highlighted.
Name:Collection and Processing of Cell Culture Media and Body Fluids Prior to Isolation of Extracellular Vesicles
Description:In this module, we will focus on the collection and processing of cell culture media and body fluids prior to isolation of extracellular vesicles. We will discuss some of the considerations and guidelines that are important during the collection of staring material and isolation of extracellular vesicles. This module will help you with the different choices, such as anticoagulants, collection time points and protein inhibitors, which are important for the outcome when working with a particular body fluid compared to conditional media or other body fluids. This module will also illustrate some example of studies on extracellular vesicles from body fluid such as blood, urine, breast milk and cerebrospinal fluid and why it is of interest to analyse extracellular vesicles from these body fluids.
Name:Methods for Isolating Extracellular Vesicles
Description:This module highlights some of the different methods used to isolate extracellular vesicles. Here the basic concepts for methods such as differential ultracentrifugation, density gradient, size exclusion and kit-based precipitation will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits.
Name:Techniques for Characterization and Quantification of Extracellular Vesicles
Description:This module highlights some of the different techniques used to characterize extracellular vesicles. Here the basic concepts for techniques such as electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be covered and some guidelines will be presented. Furthermore, this module will cover how the techniques are used in the field of extracellular vesicles as well as their limitations and benefits.
This course aims to provide the basic knowledge about extracellular vesicles (EV) a generic term including exosomes, microvesicles, microparticles, ectosomes, oncosomes, prostasomes, and many others. It covers areas such as EV history, nomenclature, biogenesis, EV cargo as well as the release and uptake mechanisms, collection and processing prior to isolation, different isolation methods, characterization and quantification techniques. This course is divided into five modules. Module 1 is an introduction to the field and will cover the nomenclature and the history of EVs. Module 2 will focus on the biogenesis, release and uptake mechanisms of EVs as well as the different EV cargos (RNA, protein, lipids). In Module 3, we will focus on the collection and processing of cell culture media and body fluids such as blood, breast milk, cerebrospinal fluid and urine prior to isolation of EVs. Module 4 and 5 will present different isolation methods and characterization/quantification techniques, respectively. Here differential ultracentrifugation, size exclusion chromatography, density gradient, kit based precipitation, electron microscopy (EM), cryo-TEM, flow cytometry, atomic-force microscopy and nanoparticle tracking analysis will be presented. The recommended prerequisites are college-freshman-level biology and biochemistry. After a completed course you should be able to: + Discuss the nomenclature and subgroups of extracellular vesicles. + Describe the RNA, protein and lipid content of extracellular vesicles. + Describe the basic concepts about the most common isolation and characterization techniques and how these techniques are used in the EV field. + State the benefits and limitations of the most common isolation and characterization techniques for extracellular vesicles. + Explain the considerations that are important during the collection and isolation of EVs from different body fluid. + Describe the release and uptake mechanisms of extracellular vesicles All lectures are given in English. Each of the five modules will be followed by an exam. All exams will be in the format of multiple choice questions. The course is organized in collaboration between the International Society for Extracellular Vesicles (ISEV), University of California Irvine (USA), University of Gothenburg (Sweden) and Pohang University of Science and Technology (South Korea).
