Engineering Genetic Circuits: Design

所在平台: Coursera

课程主页: https://www.coursera.org/learn/engineering-genetic-circuit-design

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课程简介

课程名称:工程遗传电路:设计 概述:本课程旨在介绍理解遗传电路所需的生物学和生物化学基础。课程从工程的角度出发,探讨遗传电路的设计,并对细胞及其结构进行复习。随后,课程介绍遗传元件及其标准的重要性,并讨论在电路设计中使用的遗传设备。最后两个模块涵盖了在设计过程中应用的实验技术和构建方法及原则。 课程大纲: 1. **工程师的遗传电路指南**:这一周简要介绍理解遗传电路所需的生物和生物化学知识。内容为基本概述,为后续的遗传电路建模、分析和设计奠定基础。 2. **遗传元件**:这一周强调合成生物学作为一门工程学科中标准的重要性,并介绍用于构建遗传电路的基本构建单元——遗传元件。 3. **遗传设备**:这一周介绍由多个遗传元件组合而成的遗传设备,讲解基本的组合规则以及不同类型的设备。 4. **遗传构建**:这一周介绍构建遗传电路所用的技术,包括常用的聚合酶链反应(PCR)克隆技术和DNA组装方法,以及装配不同遗传元件的构建方法。 5. **遗传电路设计原则**:这一周探讨在实施遗传电路设计时面临的实际挑战。 总之,本课程将为学习者提供坚实的基础,以便深入探讨遗传电路的设计与应用。

课程大纲

Name:An Engineers Guide to Genetic Circuits

Description:This week gives a brief introduction to the biology and biochemistry necessary to understand genetic circuits. The material covered is only a basic overview, since it is usually the topic of whole courses. It should, however, give the grounding necessary to begin studying the modeling, analysis, and design of genetic circuits.

Name:Genetic Parts

Description:This week highlights the importance of standards in synthetic biology as an engineering discipline. Furthermore, the week introduces genetic parts - the basic building used to construct genetic circuits.

Name:Genetic Devices

Description:This week introduces genetic devices, the aggregation of multiple genetic parts. Basic rules for composing, as well as different types of devices, are introduced, as well.

Name:Genetic Construction

Description:This week introduces techniques used to construct a genetic circuit. This includes commonly used methods like polymerase chain reaction for cloning and DNA assembly methods. Finally, construction methods to assemble different genetic parts are presented.

Name:Principles of Genetic Circuit Design

Description:This week introduces practical challenges in realizing genetic circuit designs.

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课程详情

This course gives an introduction to the biology and biochemistry necessary to understand genetic circuits. It starts by providing an engineering viewpoint on genetic circuit design and a review of cells and their structure. The second module introduces genetic parts and the importance of standards followed by a discussion of genetic devices used within circuit design. The last two modules cover experimental techniques and construction methods and principles applied during the design process.  

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