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所在平台: Coursera |
课程主页: https://www.coursera.org/learn/cryo-em
课程评论：没有评论
课程名称：入门冷冻电子显微镜（Cryo-EM） 课程概述：本课程覆盖冷冻电子显微镜（cryo-EM）的基本原理，内容包括电子显微镜的基本构造、傅里叶变换的介绍以及图像形成原理。在此基础上，课程进一步探讨样品准备问题、数据采集策略以及现代冷冻电子显微镜三种基本模式（切片、单粒子分析和二维晶体学）的基本图像处理工作流程。 课程理念：该课程强调概念而非数学细节，通过大量插图和示例图像进行教学。它适合任何对冷冻电子显微镜和三维电子显微镜感兴趣的人，包括没有 extensive 数学或成像物理背景的细胞生物学家或分子生物学家，以及希望扩展该领域理解的电子显微镜学实践者。课程是即将接受冷冻电子显微镜培训者或需要对该领域有初步了解以理解相关文献和会议演讲的人士的理想入门课程。 先修知识：推荐的先修课程包括大学一年级的数学、物理和生物化学。 学习进度：课程总共包含14.5小时的讲座视频，分为40个模块，每个模块平均时长20分钟。每个模块结束时都有“概念检查”问题，供学员测试所学知识。模块分为七个主要主题，一个合理的学习计划是每天学习一个主题，这样可以在一周内完成课程，同时观看几小时讲导并花费额外一小时思考概念检查问题。另一个合理的学习计划是每天学习一个模块，持续一个多月，或每周学习三模块（周一、周三和周五），持续三个月。 在开始使用冷冻电子显微镜或参与该领域后，您可能会希望重复某些模块。 课程大纲： 1. 欢迎 - 欢迎来到课程！ 2. 电镜的基本构造：电显微镜的当前、线圈、旋钮和名称 3. 傅里叶变换和倒易空间初学者 4. 图像形成 5. 生物电子显微镜的基本挑战 6. 单粒子分析 7. 切片 8. 二维晶体学 此课程为希望进入冷冻电子显微镜领域的学员提供了一个良好的基础。
Name:Welcome
Description:Welcome to the course!
Name:Currents, Coils, Knobs and Names: Basic anatomy of the electron microscope
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Name:Fourier Transforms and Reciprocal Space for Beginners
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Name:Image Formation
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Name:Fundamental Challenges in Biological EM
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Name:Single Particle Analysis
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Name:Tomography
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Name:2-D Crystallography
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This class covers the fundamental principles underlying cryo-electron microscopy (cryo-EM) starting with the basic anatomy of electron microscopes, an introduction to Fourier transforms, and the principles of image formation. Building upon that foundation, the class then covers the sample preparation issues, data collection strategies, and basic image processing workflows for all 3 basic modalities of modern cryo-EM: tomography, single particle analysis, and 2-D crystallography. Philosophy: The course emphasizes concepts rather than mathematical details, taught through numerous drawings and example images. It is meant for anyone interested in the burgeoning fields of cryo-EM and 3-D EM, including cell biologists or molecular biologists without extensive training in mathematics or imaging physics and practicing electron microscopists who want to broaden their understanding of the field. The class is perfect as a primer for anyone who is about to be trained as a cryo-electron microscopist, or for anyone who needs an introduction to the field to be able to understand the literature or the talks and conversations they will hear at cryo-EM meetings. Pre-requisites: The recommended prerequisites are college-freshman-level math, physics, and biochemistry. Pace: There are 14.5 hours of lecture videos total separated into 40 individual “modules” lasting on average 20 minutes each. Each module has at the end a list of “concept check” questions you can use to test your knowledge of what was presented. As the modules are grouped into seven major subjects, one reasonable plan would be to go through one major subject each day. That would mean watching a couple hours of lecture and spending another hour or so thinking through the concept check questions each day for a week. Another reasonable plan would be to go through one module each day for a little over a month, or even three modules a week (Monday, Wednesday, and Friday) for a 3-month term. It is likely that as you then move on to actually begin using a cryo-EM or otherwise engage in the field, you will want to repeat certain modules.
