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所在平台: Coursera |
课程主页: https://www.coursera.org/learn/chemical-biology
课程评论：没有评论
课程名称：化学生物学 课程概述：化学生物学是一个快速发展的领域，以其在实时理解生物细胞或模型生物的重要过程中的应用而受到广泛关注。该领域融合了化学、生物学和物理学的交叉学科特性，能够利用快速化学反应观察或干扰生物过程，并通过物理测定方式进行反馈。尽管化学生物学的边界广阔，其多学科的特性可能会让人感到挑战，但本课程将其核心要素进行解构，并重新组织材料，帮助学生建立实用和理论知识，掌握设计自己的化学生物学实验的能力。 课程内容概览： 1. **欢迎来到化学生物学：概念和技术** 介绍化学生物学的重要性，课程亮点及核心概念。 2. **细节中的魔鬼** 通过多色成像技术，深入探讨荧光探针的使用，显现细胞中重要过程。 3. **实时复杂参数测量的荧光测定** 讨论不同荧光技术在体外与活细胞中特定生物调控过程中的应用，聚焦于染色质调控和膜张力的调节。 4. **让蛋白质为我们服务** 探索如何利用修饰的蛋白质域，通过光学技术执行定制功能。 5. **在更大规模上发挥光的作用** 讨论近年来将光诱导化学结合于生物控制的方法，阐明各种程序的可能性。 6. **扩展视野** 学习如何使用定制探针探索复杂生物学，讨论筛选特定分子和识别目标分子的方法。 通过这一系列模块，学生不仅能进行化学生物学的理论学习，还能获取实践经验，为未来的研究打下坚实基础。
Name:Welcome to Chemical Biology: Concepts and techniques
Description:A very warm welcome to you, our new recruits! We'll start you off by acquainting you with the answers to big questions like why study chemical biology and what makes it special. We'll then show some highlights of the course that you are about to take. Finally, we want you to understand some fundamentals, so we are going to start off with some core concepts framed from the perspective of chemical biology.
Name:The Devil is in the Minutest Detail
Description:A picture used to paint a thousand words. But with the advent of multiple color imaging, microscopy can open a lens with which to view life that few other techniques are capable of. We are thus going to take you on a colorful tour that will show you specific fluorophores, explain why different fluorophores have different colors, and how to rationalize why a molecule is fluorescent. Then we will use these fluorophores in cells, to visualize important cellular processes.
Name:A ruler over time and space! Fluorescent assays to measure complex parameters in real time
Description:Here we will discuss how different fluorescent techniques have found fantastically useful applications to understand specific biological regulation processes in vitro and in live cells. We will focus on chromatin regulation and regulation of membrane tension as these are two systems which, without such techniques, we would not have the level of understanding we have today.
Name:Putting proteins to work for us
Description:The classical view of biological science is the laboratory worker working hard to understand a specific protein of interest. But through chemical biology, we have been able to employ proteins to do some of this important work for us. In this module you will learn how to deploy modified protein domains to perform custom-designed functions using light-based techniques as a readout.
Name:Making light work of it on a larger and larger scale
Description:In recent years numerous chemical biology processes have harnessed light-induced chemistry to control biology in terms of turning on or off inhibition, and exerting remote control of localization or chemical reactivity, amongst numerous other examples not covered here. We shed some light on these processes and exemplify the full spectrum of procedures possible using such approaches.
Name:Casting wide your net
Description:We've learned how complex biology is, and how resourceful chemical biologist have started to peer into this complex landscape using custom-designed probes. But given the huge complexity of biology, we often have to start from ground zero, screening for a specific molecule or identifying a target of an interesting molecule. Here we will discuss methods that are ideal for these purposes in cells and in vitro.
Chemical biology is a burgeoning field that has rapidly risen to prominence. This surge of interest has been fuelled by chemical biology’s applicability to understanding critical processes in live cells or model organisms in real time. This success has arisen because chemical biology straddles a nexus between chemistry, biology, and physics. Thus, chemical biology can harness rapid chemistry to observe or perturb biological processes, that are in turn reported using physical assays, all in an otherwise unperturbed living entity. Although its boundaries are endless, the multidisciplinary nature of chemical biology can make the field seem daunting; we beg to differ! Here, we deconstruct chemical biology into its core components, and repackage the material. In the process we build up for each student a practical and theoretical knowledge bank that will set these students on their way to understanding and designing their own chemical biology experiments. We will discuss fluorescence as a general language used to read out biological phenomena as diverse as protein localization, membrane tension, surface phenomena, and enzyme activity. We will proceed to discuss protein labeling strategies and fusion protein design. Then we will discuss larger and larger scale chemical biology mechanism and screening efforts. Highlights include a large amount of new data, tailored in the lab videos, and a large number of skilled presenters.
